MTT assay protocol
Ensure that the same volume of existing media is present for each sample. Add 50 µL of serumfree media and 50 µL of MTT solution into each well. Incubate the plate at 37°C for 3 hours. After incubation, add 150 µL of MTT solvent into each well. Wrap plate in foil and shake on an orbital shaker for 15 minutes.