ELISA AND MULTIPLEX TECHNOLOGIES FOR CYTOKINE .
ELISA. Radioimmunoassay (RIA) was first described by Yalow and Berson in 1959, a discovery for which they won the 1997 Nobel Prize in Physiology or Medicine().In search for alternative labels to replace radioactive isotopes, EnzymeLinked ImmunoSorbent Assay (ELISA) was introduced in the 1970s(10;11).In the typical double antibody sandwich ELISA (Fig. 1A), .