Restriction Enzyme Resource Guide
A. Restriction Enzyme Classifiion. Restriction endonucleases are egorized into one of four general groups (Types I, II, III, and homing endonucleases based on their subunit structure, cofactor requirements, specificity of cleavage, and associated methylase activity ().References 110 provide reviews of each restriction enzyme type as follows: Type II and Type II subclasses .
